RXFP1 (relaxin family peptide receptor 1) is a G-protein-coupled receptor whose activation is commonly measured in vitro by cAMP accumulation and downstream pathway reporters. This guide outlines how RXFP1 signaling is studied.
What RXFP1 is
RXFP1 is a GPCR activated by relaxin and by relaxin-derived research peptides such as B7-33. Activation triggers intracellular signaling that cell-based assays can quantify.
Core readouts
- cAMP accumulation — a common readout of RXFP1 activation.
- Pathway reporters — reporter-gene or marker assays for downstream signaling.
- Binding assays — affinity at the receptor.
Designing the assay
RXFP1-expressing cell lines with vehicle controls, a reference agonist, and replicates give interpretable concentration-response data (EC50). Concentration is expressed in molarity, never a dose.
Related research guides
- B7-33: relaxin-receptor (RXFP1) research profile
- B7-33 structure & the relaxin family
- Sourcing B7-33 (COA, purity)
- B7-33 vs full-length relaxin
Research-use note
All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.
