Lab bench instrument screens showing an HPLC chromatogram with one dominant peak beside a deconvoluted mass spectrum, representing COA purity and identity verification.
Mindful Research

Sourcing GLP-3 RT as a Research Material: COA, Purity and Triple-Agonist Identity Verification

By: Mindful Research Team3 min read

Key takeaways

  • The name GLP-3 RT is an unregulated label, so identity must come from analytics.
  • Look for CAS 2381089-83-2, a lot-matched COA, HPLC purity, MS mass near 4731 Da, net content and endotoxin.
  • A salt-form weight near 4845 can describe the same peptide; the report should state its basis.
  • Identity matters more for a triple agonist because truncations or missing lipid change the mass.
  • For in vitro research use only.

When sourcing GLP-3 RT (the GLP-3RT peptide, retatrutide, listed here as RT-GLP3) as a research material, the name on the vial is not enough: you need the CAS number (2381089-83-2), a batch-specific certificate of analysis with HPLC purity, a mass-spectrometry identity result near 4731 Da, net peptide content and an endotoxin result.

"GLP-3 RT", "GLP3RT" and "GLP-3 peptide" are unregulated catalog labels for one compound. That makes verification, not branding, the basis of a purchasing decision. This guide covers the triple-agonist-specific checks and points to the deeper method guides rather than repeating them.

Why isn't the product name enough?

Catalog numbering such as GLP-1, GLP-2 and GLP-3 is a supplier convention, not a standard. Two vials with the same label can differ in sequence, in whether the lipid side chain is present, or in salt form. Only batch analytics can distinguish them. The background on the label itself is in the hub guide listed below.

What should a GLP-3 RT report show?

Check What to look for Red flag
CAS number 2381089-83-2 on the spec and report Missing, or a different CAS
Lot traceability Batch code on the report matches the vial Generic report with no lot number
HPLC purity Dated chromatogram with a dominant main peak and stated method Purity percentage with no chromatogram
MS identity Deconvoluted mass near 4731 Da (free peptide) No mass result, or a mass that does not match
Net peptide content Stated net content per vial Only a label amount, no measured content
Endotoxin Measured result for the batch No endotoxin line

The line-by-line reading of a report is in Reading a Retatrutide COA, Line by Line, and the HPLC and MS method detail is in How to Verify Retatrutide Purity.

Why does identity matter more for a triple agonist?

The three-receptor profile depends on the modified residues and the C20 fatty-diacid side chain. A truncated sequence, a deletion product or a species missing the lipid moiety has a different mass and shows up as a separate species in an MS trace, so a clean identity result protects the receptor-specific readouts you plan to run. The mass-spectrometry specifics for this compound are in Retatrutide Identity Confirmation by Mass Spectrometry.

Which molecular weight should you compare against?

The free-peptide average mass is about 4731 g/mol (C221H342N46O68). A listing that quotes a figure near 4845 is consistent with a trifluoroacetate salt form, because each TFA adds about 114 Da. Both can describe the same peptide; the report should say which basis it uses.

Where to source GLP-3 RT as a research material

Happy Peptides supplies RT-GLP3 (retatrutide) as a lyophilized research material in 10 mg, 20 mg and 30 mg vials. Recent batch reports listed on the product page cover identity, purity, net content and endotoxin results, and every batch report is searchable by batch number in the certificate of analysis library.

Related research guides

Research-use note

All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.

Frequently asked questions

What should a GLP-3 RT certificate of analysis show?

CAS number 2381089-83-2, a lot number matching the vial, a dated HPLC chromatogram and purity, a mass-spectrometry identity result, net peptide content and an endotoxin result. Reading a retatrutide COA

What mass confirms GLP-3 RT identity?

The free-peptide average mass is about 4731 Da (C221H342N46O68). A listing near 4845 is consistent with a trifluoroacetate salt form, and the report should state which basis it uses. Retatrutide identity by mass spectrometry

Why isn't the name GLP-3 RT enough to confirm identity?

GLP-3 RT, GLP3RT and GLP-3 peptide are unregulated catalog labels, so only the CAS number and batch analytics show what is in the vial.

Where can I read more about verifying purity?

The HPLC and mass-spectrometry method guide covers chromatogram reading, purity percentages and what a clean identity result looks like. How to verify retatrutide purity

Where can I find batch reports for RT-GLP3?

Batch reports for each lot are listed on the product page and searchable by batch number in the certificate of analysis library. COA library