A cjc 1295 ipamorelin blend sourced as a research material should arrive with a Certificate of Analysis that reports each peptide separately: an HPLC purity value for ipamorelin and one for CJC-1295 no DAC, a mass-spectrometry identity for each, and the mass ratio in the vial, which for the catalog product is 5 mg ipamorelin to 5 mg CJC-1295 no DAC. This guide lists what to check on a two-component COA, why a single purity number is not enough, and how to turn the 5/5 mg vial into working stocks expressed in both mg/mL and molarity.
What should a blend COA report?
A single-peptide COA has one identity and one purity line. A blend needs two of each, plus the ratio. The Ipamorelin/CJC-1295 (no DAC) listing states that component identities, ratios and lot-specific purity values are printed on the certificate; the checklist below is what to look for.
| COA item | Ipamorelin | CJC-1295 no DAC |
|---|---|---|
| Formula | C38H49N9O5 | C152H252N44O42 |
| MW (g/mol) | 711.9 | 3367.95 |
| CAS | 170851-70-4 | 863288-34-0 |
| MS signature | [M+H]+ about 712.9; [M+2H]2+ about 356.9 | [M+4H]4+ about 843.0; [M+3H]3+ about 1123.6 |
| HPLC | Separate peak and purity value | Separate peak and purity value |
| Stated mass | 5 mg | 5 mg |
Why is one purity number not enough?
A blend reported as a single "99 percent" figure could mean each peptide is 99 percent pure, or that the two peaks together account for 99 percent of the chromatogram with no information about their ratio. Those are different statements. Ask for the individual purity values and for the area ratio or a gravimetric statement that supports the 5/5 mg claim. If only a combined value is given, the ratio can be estimated from the two HPLC peak areas after correcting for the different extinction coefficients of a 5-residue and a 29-residue peptide at 214 or 220 nm, but a vendor-reported ratio is better. The general approach is in reading a COA line by line and HPLC for peptides: how purity is measured.
How do you confirm both identities?
Two mass spectra, or one spectrum with two clearly assignable species. Ipamorelin at 711.9 g/mol is seen as a singly or doubly charged ion; CJC-1295 no DAC at 3367.95 g/mol appears as a multiply charged envelope. The two cannot be confused, and a DAC-containing CJC-1295 would shift the envelope by about 279 units, as described in CJC-1295 with DAC vs no DAC. See mass spectrometry for peptide identity for how to read the envelope.
Reconstitution math for the 5 mg / 5 mg vial
Reconstituting the entire vial in a given volume of reconstitution solution gives the following stock concentrations for each component simultaneously:
| Volume added | Ipamorelin | CJC-1295 no DAC | Molar ratio (Ipa : CJC) |
|---|---|---|---|
| 1 mL | 5 mg/mL = 7.02 mM | 5 mg/mL = 1.48 mM | 4.73 : 1 |
| 2 mL | 2.5 mg/mL = 3.51 mM | 2.5 mg/mL = 0.742 mM | 4.73 : 1 |
| 4 mL | 1.25 mg/mL = 1.76 mM | 1.25 mg/mL = 0.371 mM | 4.73 : 1 |
The molar ratio is fixed at about 4.7 to 1 whatever the volume, because it is set by the masses in the vial. Working solutions in the nanomolar to micromolar range are made by serial dilution of the stock in assay medium, following peptide concentration and reconstitution math. For an equimolar design, reconstitute the single-component CJC-1295 no DAC and ipamorelin vials separately.
Storage and handling of the blend
Both components are stable as a lyophilized powder when kept cold, dry and dark. After reconstitution, aliquot into single-use volumes and freeze; a 29-residue amphipathic peptide adsorbs to plastic, so low-binding tubes and a carrier-protein-free buffer only for short periods are sensible. Avoid repeated freeze-thaw.
Supplier questions to ask
- Are purity and identity reported per component?
- Is the 5/5 mg ratio stated on the COA, and how was it determined?
- Is the CJC-1295 the no-DAC form (formula C152H252N44O42)?
- Is an endotoxin value given, if the material will be used in cell culture?
The general supplier checklist is in how to evaluate a research-compound supplier, and the blend's receptor pharmacology in the CJC-1295 + ipamorelin blend profile.
Related research guides
- CJC-1295 + ipamorelin blend: in-vitro profile
- CJC-1295 with DAC vs no DAC: structure and stability differences
- Ipamorelin peptide: pentapeptide structure and stability
- Sourcing ipamorelin as a research material: COA and purity
- Reading a Certificate of Analysis line by line
- Peptide concentration and reconstitution math
Research-use note
All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.
