Reconstituting a T-GLP2 (tirzepatide) vial to a known mg/mL stock is a mass-over-volume calculation, C = m / V: for example 10 mg in 1 mL gives 10 mg/mL, and 30 mg in 3 mL gives 10 mg/mL.
How do you calculate a T-GLP2 stock concentration?
Divide the vial mass by the solvent volume added. T-GLP2 is offered in 10, 30, 60 and 100 mg vials; the table pairs each with common volumes. The arithmetic is identical for any peptide and is walked through in the peptide concentration and reconstitution math guide.
| Vial | Solvent volume | Concentration (mg/mL) | Approx. molarity |
|---|---|---|---|
| 10 mg | 1 mL | 10 mg/mL | ~2.08 mM |
| 10 mg | 2 mL | 5 mg/mL | ~1.04 mM |
| 30 mg | 2 mL | 15 mg/mL | ~3.12 mM |
| 30 mg | 3 mL | 10 mg/mL | ~2.08 mM |
| 60 mg | 3 mL | 20 mg/mL | ~4.15 mM |
| 100 mg | 5 mL | 20 mg/mL | ~4.15 mM |
| 100 mg | 2 mL | 50 mg/mL | ~10.39 mM |
How do you convert mg/mL to molarity?
Molarity equals the concentration in g/L divided by the molecular weight in g/mol. Tirzepatide’s molecular weight is ~4813 g/mol, so a 10 mg/mL stock is 10 g/L ÷ 4813 g/mol ≈ 2.08 mM. Because tirzepatide is a large peptide, a given mg/mL translates to a relatively low molarity compared with small molecules — useful to keep in mind when matching assay concentrations across compounds and when preparing serial dilutions and working solutions.
What solvent and handling suit T-GLP2?
Tirzepatide ships as a white lyophilized powder and is reconstituted for in vitro use in an aqueous-compatible reconstitution solution; keep the vial cold and sealed until use and aliquot the stock to limit freeze-thaw cycles. Match the vial size to your target working concentration on the T-GLP2 listing.
Related research guides
- Tirzepatide Peptide (T-GLP2): Identity and Structure
- Sourcing T-GLP2 (Tirzepatide) Research Material: COA & Purity
- GLP-2 the Hormone vs the T-GLP2 Catalog Label
- Stock and Working Solutions: Serial Dilution for In-Vitro Assays
Research-use note
All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.
