Scientist inspecting a lyophilized peptide vial before reconstitution with buffer, illustrating in vitro stock concentration math
Mindful Research

BPC-157 10 mg Vial: Reconstitution and Stock Concentration Math (In Vitro)

By: Mindful Research Team2 min read

Key takeaways

  • A 10 mg BPC-157 vial in 2 mL = 5 mg/mL stock.
  • 5 mg/mL equals about 3.52 mM using MW 1419.5.
  • mg/mL = vial mass / solvent volume; mM = mg/mL / MW x 1000.
  • Reconstitute with bacteriostatic reconstitution solution.
  • For in vitro research use only.

Reconstituting a 10 mg BPC-157 vial in 2 mL of solvent gives a stock of 5 mg/mL, which is about 3.52 mM given the peptide's molecular weight of 1419.5 g/mol.

How is the concentration calculated?

Stock concentration in mg/mL is simply the vial mass divided by the solvent volume added. For a 10 mg vial: 10 mg ÷ 2 mL = 5 mg/mL; 10 mg ÷ 1 mL = 10 mg/mL; 10 mg ÷ 4 mL = 2.5 mg/mL. Choose the volume that puts your working range on scale for your assay.

How do you convert to molarity?

Molar concentration = (mg/mL) ÷ MW × 1000, in mM. With MW 1419.5: a 5 mg/mL stock is 5 ÷ 1419.5 × 1000 ≈ 3.52 mM. A 10 mg/mL stock is ≈ 7.04 mM. Working dilutions are then prepared by serial dilution into assay buffer.

What solvent is used?

Lyophilized BPC-157 is typically reconstituted with bacteriostatic reconstitution solution; the full method is in our concentration and reconstitution math guide. Research-grade BPC-157 dissolves readily in aqueous solvent.

All figures here are concentration math for in-vitro stock preparation and are not a recommendation for any use in humans or animals.

Related research guides

Research-use note

All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.

Frequently asked questions

What concentration is a 10 mg BPC-157 vial?

Reconstituted in 2 mL it is 5 mg/mL, which is about 3.52 mM given MW 1419.5. reconstitution math

How do you convert BPC-157 mg/mL to molarity?

Divide mg/mL by the molecular weight 1419.5 and multiply by 1000 to get mM.

What solvent reconstitutes BPC-157?

Bacteriostatic reconstitution solution is commonly used for the lyophilized powder. reconstitution solution

Continue reading

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