3D ball-and-stick render of a fifteen-residue pentadecapeptide chain, illustrating sequence and structure for in vitro research
Mindful Research

BPC-157 Peptide: Pentadecapeptide Sequence, Structure and In-Vitro Research Profile

By: Mindful Research Team2 min read

Key takeaways

  • BPC-157 is a synthetic 15-residue peptide (sequence GEPPPGKPADDAGLV).
  • Molecular formula C62H98N16O22, MW ~1419.5, CAS 137525-51-0, PubChem CID 9941957.
  • Written variously as BPC 157, bp 157 peptide, or BPC 157 peptides.
  • Studied in vitro via NO-system, angiogenesis and cell-migration assays.
  • For in vitro research use only.

BPC-157 is a synthetic pentadecapeptide, a 15-amino-acid chain (sequence GEPPPGKPADDAGLV) corresponding to a partial fragment of a protein found in gastric juice, studied in vitro as a research material.

The BPC-157 peptide is one of the most searched research peptides, and the name appears written several ways — BPC-157, BPC 157, bp 157 peptide, or the plural BPC 157 peptides. All refer to the same compound: a short, fully defined sequence rather than a full-length protein.

What is BPC-157?

BPC-157 ("body protection compound-157") is a stable pentadecapeptide. Its molecular formula is C62H98N16O22, its monoisotopic/average molecular weight is approximately 1419.5, and its CAS number is 137525-51-0 (PubChem CID 9941957). Because it is only fifteen residues long, it is prepared by solid-phase peptide synthesis and characterized by HPLC and mass spectrometry like other short peptides.

What does the sequence look like?

The one-letter sequence is Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (GEPPPGKPADDAGLV). The three consecutive prolines near the N-terminus contribute to the conformational rigidity that researchers associate with its stability in aqueous and acidic conditions.

How is BPC-157 studied in vitro?

In cell-based research, BPC-157 is most often discussed in the context of nitric-oxide (NO) system signaling and angiogenesis-related assays, as well as fibroblast and endothelial cell-migration readouts such as the scratch assay. These are mechanistic, laboratory models; this profile describes assay context only and makes no claims about effects in humans or animals.

How is identity and purity confirmed?

Compound identity is confirmed by mass spectrometry against the expected mass for C62H98N16O22, and purity is measured by reversed-phase HPLC. A certificate of analysis should report both. See our guides on mass spectrometry for peptide identity and reading a COA line by line. Research-grade BPC-157 is supplied as a lyophilized powder for reconstitution with reconstitution solution.

Related research guides

Research-use note

All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.

Frequently asked questions

What is the BPC-157 peptide?

BPC-157 is a synthetic pentadecapeptide (15 residues, sequence GEPPPGKPADDAGLV) corresponding to a partial fragment of a gastric protein, studied in vitro as a research material. BPC-157 research material

What is the molecular formula of BPC-157?

Its molecular formula is C62H98N16O22 with a molecular weight of about 1419.5 and CAS number 137525-51-0.

Is BPC-157 a peptide or a protein?

It is a peptide: at fifteen residues it is a short chain made by solid-phase synthesis, not a full-length folded protein.

How is BPC-157 identity confirmed?

By mass spectrometry against the expected mass for C62H98N16O22 and by reversed-phase HPLC for purity. mass spec for identity

Continue reading

Two research vials of lyophilized peptide powder beside a volumetric flask and pipette, illustrating in vitro stock concentration preparationBPC-157, TB-500 Blend: Vial Reconstitution and Stock Concentration Math (In Vitro) Reconstituting the 10 mg / 10 mg BPC-157, TB-500 vial in a single aqueous volume gives each peptide its own molar concentration—both sit at... Scientist in lab coat reviewing a two-peak HPLC chromatogram to verify a two-component peptide blend for in vitro researchSourcing a BPC-157, TB-500 Blend: Two-Component COA and Ratio Verification A credible BPC-157, TB-500 blend COA must confirm both peptides independently—each resolved by mass spectrometry at its expected mass and each quantified... 3D render of two peptide chains side by side, illustrating blend composition and ratio studied in parallel in vitroBPC-157, TB-500 Blend Composition and Ratio: Why Two Peptides Are Studied in Parallel (In Vitro) In the BPC-157, TB-500 blend the two peptides are present at a fixed 1:1 mass ratio (10 mg each in current lots), and...