If you are looking for MOTS-c for sale as a research material, judge the supplier by its batch-specific certificate of analysis: HPLC purity, mass-spectrometry identity near 2174.6 g/mol, an endotoxin result, and CAS 1627580-64-6 matched to the lot. This guide covers what to check when sourcing MOTS-c for in vitro work.
What should a MOTS-c certificate of analysis show?
| COA item | What to look for |
|---|---|
| Lot number | Matches the vial you receive, with a test date |
| HPLC purity | Chromatogram with one dominant peak and a stated percent purity |
| Mass spectrometry | Observed mass consistent with C101H152N28O22S2 (2174.6 g/mol average), for example [M+2H]2+ near 1088.3 and [M+3H]3+ near 725.9 |
| Identifiers | MOTS-c, CAS 1627580-64-6, 16-residue sequence MRWQEMGYIFYPRKLR |
| Endotoxin | A reported result for the lot, important for cell-based assays |
| Form | Lyophilized powder, with net peptide content if reported |
How do you read HPLC purity and the MS result?
HPLC purity is the share of the main peak's area among all detected peaks; a clean dominant peak supports a high-purity claim. Mass spectrometry then confirms that the main peak is the right molecule, which matters for a 16-residue peptide with two methionines that can show oxidized (+16 Da) species. Both are needed, because a pure peak of the wrong peptide is still the wrong material. For a line-by-line walkthrough, see reading a certificate of analysis.
Why does endotoxin matter for MOTS-c assays?
Endotoxin can activate stress signaling in cultured cells, which can confound AMPK and other metabolic readouts. A reported endotoxin result for the lot lets you rule that out. The assay designs themselves are described in AMPK and folate-cycle assays for MOTS-c in vitro.
What are the red flags when buying a research peptide?
- A generic COA with no lot number or date.
- A purity figure with no chromatogram.
- No mass-spectrometry data, or a CAS number that does not match.
- No endotoxin result for material intended for cell culture.
- Marketing that implies human or animal use, which a genuine research-use-only supplier does not make.
Where can you source MOTS-c as a research material?
Happy Peptides supplies MOTS-c (CAS 1627580-64-6, about 99% by HPLC) as a lyophilized research material in 10 mg and 40 mg vials, with batch documentation, for in vitro use only. Before ordering, you can review the compound's chemistry in the MOTS-c quick-reference profile.
Find a COA: MOTS-c batch reports are listed in our certificate of analysis library, searchable by batch number.
Related research guides
- MOTS-c 10 mg vial: stock concentration math
- MOTS-c vs SS-31: two mitochondria-directed peptides
- Sourcing SS-31 as a research material: COA and purity
Research-use note
All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.
