The GLOW 70 mg vial contains 10 mg BPC-157, 10 mg TB-500 and 50 mg GHK-Cu; reconstituted in 1 mL it gives a stock of 10 mg/mL BPC-157 (about 7.0 mM), 10 mg/mL TB-500 (about 2.0 mM) and 50 mg/mL GHK-Cu (about 124 mM), and every figure halves in 2 mL and quarters in 4 mL. This guide sets out the vial specification and works the concentration arithmetic for each component so that a blend experiment can be reported in molarity.
What is the GLOW 70 mg vial specification?
GLOW is listed as a single 70 mg vial. The 70 mg is the nominal sum of three listed component masses, not the mass of one peptide:
| Component | Listed mass per vial | Residues | CAS number | Formula | MW (g/mol) |
|---|---|---|---|---|---|
| BPC-157 | 10 mg | 15 | 137525-51-0 | C62H98N16O22 | 1419.5 |
| TB-500 | 10 mg | 43 | 77591-33-4 | C212H350N56O78S | 4963 |
| GHK-Cu | 50 mg | 3 (copper complex) | 89030-95-5 | C14H23CuN6O4 | 402.92 |
The lot-specific component ratio is stated on the Certificate of Analysis, and that certificate, not the listing, is the number to use when the ratio matters for an experiment.
Why does a blend need per-component math?
The three molecular weights span more than an order of magnitude: 402.92 g/mol for GHK-Cu, 1419.5 for BPC-157 and about 4963 for TB-500. Equal masses are therefore very unequal numbers of molecules, and a figure such as "70 mg/mL" says nothing useful about what a cell in culture is exposed to. The general method, mass divided by molecular weight divided by volume, is the same as in the peptide concentration and reconstitution math guide; a blend simply runs it three times.
What is the stock concentration at 1, 2 and 4 mL?
| Volume added | BPC-157 (1419.5 g/mol) | TB-500 (4963 g/mol) | GHK-Cu (402.92 g/mol) |
|---|---|---|---|
| 1 mL | 10 mg/mL, 7.04 mM | 10 mg/mL, 2.01 mM | 50 mg/mL, 124 mM |
| 2 mL | 5 mg/mL, 3.52 mM | 5 mg/mL, 1.01 mM | 25 mg/mL, 62.0 mM |
| 4 mL | 2.5 mg/mL, 1.76 mM | 2.5 mg/mL, 0.50 mM | 12.5 mg/mL, 31.0 mM |
Worked example for BPC-157 in 2 mL: 10 mg ÷ 1419.5 g/mol = 7.04 µmol; 7.04 µmol ÷ 2 mL = 3.52 mM. The same two steps give every cell in the table. Because 70 mg of solids in 1 mL is a dense solution, 2 mL or more of reconstitution solution is the more usual choice for a blend of this size; the ratio between components is identical at any volume.
How do you reach a working concentration?
Pick the component whose molarity drives the experimental design and dilute for that one; the other two follow at the fixed ratio. From the 2 mL stock, a 1 : 1000 dilution into assay medium gives 3.5 µM BPC-157, 1.0 µM TB-500 and 62 µM GHK-Cu. If the design needs the three peptides at matched molarity, the blend cannot provide it; add single components from the separate BPC-157, TB-500 and GHK-Cu vials instead, as discussed in the GLOW blend profile.
How should a blend concentration be reported?
As three numbers. A methods section that says "GLOW at 10 µg/mL" is not reproducible, because another laboratory cannot recover the molarity of any component from it. State each component's final molarity, the lot number, and the reconstitution volume. The conventions are collected in multi-peptide research blend terminology.
Handling notes
Add the solvent down the side of the vial, swirl, and wait until the blue solution is clear and uniform. Aliquot the stock so that each aliquot is thawed once, store frozen, and prepare working dilutions fresh. The copper complex gives the solution its colour; a colourless solution would suggest the wrong product and should be checked against the COA before use.
Related research guides
- GLOW peptide blend: components and in-vitro dermal research profile
- What is GLOW peptide? Components, ratios and CAS numbers
- KLOW 80 mg: copper-complex blue powder handling and stability
- Peptide concentration and reconstitution math for the lab
- Concentration calculator: mg/mL and molarity explained
Research-use note
All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.
