Research vial of lyophilized peptide in front of a single-peak HPLC purity trace, representing COA review for in vitro use
Mindful Research

Sourcing TB-500 as a Research Material: COA and Purity

By: Mindful Research Team1 min read

Key takeaways

  • Source TB-500 on analytical evidence: HPLC purity, MS identity, and net content.
  • MS identity should match the full-length ~4963 g/mol peptide, not the short fragment.
  • Purity is an HPLC result; appearance tells you nothing.
  • Check lot-specific documentation and storage on arrival.
  • For in vitro research use only.

Sourcing TB-500 as a research material comes down to analytical evidence: a certificate of analysis showing HPLC purity, mass-spectrometry identity consistent with the ~4963 g/mol full-length peptide, and net peptide content.

What should a TB-500 COA show?

A useful certificate for TB-500 reports HPLC purity (commonly ~99%), an identity confirmation by mass spectrometry, and net content. Reading each line is covered in the line-by-line COA guide. Identity matters especially here because the "fragment versus full peptide" ambiguity, explained in the TB-500 identity profile, can only be settled by mass data.

How is purity actually measured?

Purity is a chromatography result, not a visual one: reversed-phase HPLC separates and quantifies the main peak against impurities. A powder looking white tells you nothing about purity.

What else belongs in a sourcing check?

Lot-specific documentation, consistent net content, and sensible storage on arrival. Labs sourcing TB-500 often also evaluate the two-component BPC-157, TB-500 blend and single BPC-157 against the same COA standard.

Related research guides

Research-use note

All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.

Frequently asked questions

What should a TB-500 certificate of analysis include?

HPLC purity, a mass-spectrometry identity confirmation matching the ~4963 g/mol full-length peptide, and net peptide content. Reading a COA

Can I judge TB-500 purity by looking at it?

No; purity is measured by HPLC, not appearance. HPLC for peptides

Where can I source TB-500 as a research material?

From suppliers that provide lot-specific COAs; see the TB-500 product page. TB-500