KLOW is a co-formulated research blend of four synthetic peptides, BPC-157 (10 mg, CAS 137525-51-0), TB-500 (10 mg, CAS 77591-33-4), GHK-Cu (50 mg, CAS 89030-95-5) and KPV (10 mg, CAS 67727-97-3), supplied together as a single 80 mg lyophilized vial for in vitro research in fibroblast, extracellular-matrix and cytokine-signaling cell models. This profile covers what is in the vial, how the four components differ, how the blend is framed for cell-culture work, and what the Certificate of Analysis should show.
What is the KLOW peptide blend?
KLOW is a catalog name for a four-component blend. The vial contains four separately synthesized peptides combined before lyophilization, so the powder is a physical mixture and each component keeps its own identity. The listed masses are 10 mg BPC-157, 10 mg TB-500, 50 mg GHK-Cu and 10 mg KPV, for a nominal 80 mg of peptide; the lot-specific ratio is on the certificate. Queries that use the bare word "KLOW", or "KLOW blend", refer to this same composition.
| Component | Listed mass per vial | Residues | CAS number | Formula | MW (g/mol) |
|---|---|---|---|---|---|
| BPC-157 | 10 mg | 15 | 137525-51-0 | C62H98N16O22 | 1419.5 |
| TB-500 | 10 mg | 43 | 77591-33-4 | C212H350N56O78S | 4963 |
| GHK-Cu | 50 mg | 3 (copper complex) | 89030-95-5 | C14H23CuN6O4 | 402.92 |
| KPV | 10 mg | 3 | 67727-97-3 | C16H30N4O4 | 342.43 |
The copper complex GHK-Cu makes the lyophilized powder pale blue. The colour confirms copper, and nothing more; identity of the other three components rests on mass spectrometry.
What does each component contribute?
Four different classes of molecule. BPC-157 is a 15-residue linear peptide derived from a gastric-juice protein sequence, covered in the BPC-157 and TB-500 primer. TB-500 is synthetic thymosin beta-4, a 43-residue actin-binding polypeptide. GHK-Cu is the Gly-His-Lys tripeptide complexed with copper(II), whose chemistry is described in GHK-Cu copper coordination chemistry. The fourth component, KPV, is the tripeptide Lys-Pro-Val, the C-terminal fragment (residues 11-13) of alpha-melanocyte-stimulating hormone, studied in cell models of cytokine signaling; its own profile is KPV: a tripeptide in inflammation research. Each component is broken out further in what is KLOW peptide.
By molarity the blend is dominated by its two tripeptides. Reconstituted in 1 mL, it is roughly 7.0 mM BPC-157, 2.0 mM TB-500, 124 mM GHK-Cu and 29 mM KPV. Experiments that compare the blend with single components are therefore designed by molarity of each peptide, never by milligrams of powder; the numbers are worked in the KLOW 80 mg handling and concentration guide.
How is KLOW framed for in vitro research?
The three GLOW components are individually studied in connective-tissue cell models, and KPV adds a cytokine-signaling axis: it is the fragment studied for its interaction with NF-kB-dependent readouts in cultured cells. The in vitro questions for the blend are therefore mechanistic and two-fold: fibroblast and matrix readouts (collagen and fibronectin synthesis, metalloproteinase expression, migration in a scratch assay) and cytokine-pathway readouts (reporter activity, cytokine secretion by ELISA) in the same or parallel cultures. Assay designs are described in collagen and fibroblast assays and cytokine signaling pathways in vitro. The blend is a reagent for those assays; no outcome claim is made for it.
Two controls matter for a four-component vial: a copper-only control at the GHK-Cu molarity, to separate metal-ion effects from peptide effects, and single-peptide arms at the molarity each peptide has in the blend, to attribute any readout to a component.
What should the KLOW COA show?
Four HPLC purities and four mass-spectrometry identities, one per component, with observed masses near 1419, 4963, 403 and 342 g/mol, plus the lot ratio. A single purity figure for the vial cannot show that all four peptides are present. The checks are listed in verifying a multi-component blend COA and the sourcing questions in sourcing KLOW as a research material.
How does KLOW relate to GLOW?
GLOW is the three-component sibling without KPV. Everything else, including the masses of the three shared peptides, is the same. The comparison is set out in GLOW vs KLOW, and GLOW's own profile is at GLOW peptide blend.
Reconstitution and storage in brief
Reconstitute with reconstitution solution, swirl until the blue solution is uniform, aliquot, and store the stock frozen with each aliquot thawed once. Working dilutions are prepared fresh in assay medium.
Related research guides
- What is KLOW peptide? Component-by-component breakdown
- GLOW vs KLOW: what KPV adds to the blend
- Sourcing KLOW: per-component COA and purity
- KLOW 80 mg: copper-complex blue powder handling and stability
- GLOW peptide blend: components and in-vitro dermal research profile
- KPV: a tripeptide in inflammation research
Research-use note
All materials discussed are supplied for in vitro laboratory research use only, and are not for human or animal use.
